This title presents cutting-edge quantitative phase imaging techniques and their applications. Filled with unique, full-color images taken by advanced quantitative phase imaging (QPI), "Quantitative Phase Imaging of Cells and Tissues" thoroughly explores this innovative technology and its biomedical applications. An introductory background on optical imaging and traditional optical microscopy is included to illustrate concept development. The book explains how various visualization modalities can be obtained by numerical calculations. This authoritative resource reveals how to take full advantage of the unprecedented capabilities of QPI, such as rendering scattering properties of minute subcellular structures and nanoscale fluctuations in live cells. Coverage includes: Groundwork; Spatiotemporal field correlations; Image characteristics; Light microscopy; Holography; Point scanning QPI methods; Principles of full-field QPI; Off-axis full-field methods; Phase-shifting techniques; Common-path methods; White light techniques; Fourier transform light scattering (FTLS); and, Current trends in QPI.
Chapter 1. Introduction Chapter 2. Groundwork Chapter 3. Spatiotemporal Field Correlations Chapter 4. Image Characteristics Chapter 5. Light Microscopy Chapter 6. Holography Chapter 7. Point-Scanning QPI Methods Chapter 8. Principles of Full-Field QPI Chapter 9. Off-Axis Methods Chapter 10. Phase-Shifting Methods Chapter 11. Common-Path Methods Chapter 12. White Light Methods Chapter 13. Fourier Transform Light Scattering Chapter 14. Current Trends in Methods Chapter 15. Current Trends in Applications Appendix A. Complex Analytic Signals Appendix B. The Two-Dimensional and Three-Dimensional Fourier Transform Appendix C. QPI Artwork Index