| Ch. 1 | | Overview : developments in DNA sequencing by Keith R. Mitchelson and David B. Hawkes and Rustam Turakulov and Artem E. Men | | 3 |
| Ch. 2 | | Chip capillary electrophoresis and total genetic analysis systems by Qiang Xiong and Jing Cheng | | 45 |
| Ch. 3 | | Comparative sequence analysis by MALDI-TOF mass spectrometry - utilizing the known to discover the new by Mathias Ehrich and Franz Hillenkamp and Dirk van den Boom | | 97 |
| Ch. 4 | | Advances in dye-nucleotide conjugate chemistry for DNA sequencing by Shiv Kumar and Carl W. Fuller | | 119 |
| Ch. 5 | | The 454 life sciences picoliter sequencing system by Marcel Margulies and Thomas P. Jarvie and James R. Knight and Jan Fredrik Simons | | 153 |
| Ch. 6 | | An integrated system for DNA sequencing by synthesis by John R. Edwards and Dae Hyun Kim and Jingyue Ju | | 187 |
| Ch. 7 | | Single-molecule fluorescence microscopy and its applications to single-molecule sequencing by cyclic synthesis by Benedict Hebert and Ido Braslavsky | | 209 |
| Ch. 8 | | Rapid DNA sequencing by direct nanoscale reading of nucleotide bases on individual DNA chains by James Weifu Lee and Amit Meller | | 245 |
| Ch. 9 | | A single molecule system for whole genome analysis by Shiguo Zhou and Jill Herschleb and David C. Schwartz | | 265 |
| Ch. 10 | | Sequencing aided by mutagenesis facilitates the De Novo sequencing of megabase DNA fragments by short read lengths by Jonathan M. Keith and David B. Hawkes and Jacinta C. Carter and Duncan A. E. Cochran and Peter Adams and Darryn E. Bryant and Keith R. Mitchelson | | 303 |
| Ch. 11 | | Genome sequencing and assembly by Annette McGrath | | 327 |
| Ch. 12 | | Valid recovery of nucleic acid sequence information from high contamination risk samples - ancient DNA and environmental DNA by George A. Kowalchuk and Jeremy J. Austin and Paul S. Gooding and John R. Stephen | | 357 |